Toluene diisocyanate (TDI) and plasma protein binding


Bags from the bag to determine the drugs outside the free diffusion time required to reach equilibrium. 1.4 Determination of linear relations and the recovery obtained in human plasma proteins and buffer all six copies, each 2ml, by adding different amounts of I were mixed to make into a series of concentration, set the refrigerator for about 50h, according to the above experimental methods to extract , dilution, determination of content. Parallel to the repeated 5 times. e peak area of ​​concentration regression analysis, with the simultaneous determination of the concentration of methylene chloride solution of the same I I were calculated recovery. 1.5 data of protein binding: a balance to be spread I after the dialysis bags were measured I concentrations in plasma (total concentration Dt) and I bags outside the buffer concentration (free concentration Df). cording to the formula (1) obtain the plasma protein binding of I [6]. Fb = (Dt-Df) / Dt × 100% (1) 2 2.1 linear relationship between the results of a buffer solution and recovery I and I a plasma processing method according to the above determination, to observe the chromatogram in Figure 2,canada goose jakke, Figure 3. at extracted by the protein binding, I's structure has not changed. e peak area of ​​concentration regression analysis, the concentration of abscissa x, the peak area for the vertical axis Y, the regression equation: Y = 79840X +157435, R = 0.9998. In Table 1. at the concentration of 1.425.2nl0l, L has a good linear relationship,moncler jacken, shown in Figure 1. Table 1I in the linear range of plasma (n = 5) ChineseJoumalofClinicalPracticalMedicine,abercrombie fitch madrid, June, 2005, Vo1.6. No. 6.8 million 60000O old 40o000 plastic 20O0000) ● ● E eleven =:] = 'blue stem:;: 0246 concentration (mol children) Figure 1I in the linear range of the plasma blank plasma by adding 2 1.2mol/LI Figure 3 Chromatogram of blank plasma chromatogram adding 2.8mol/LI linear measurement range,mulberry outlet, we can see from Table 1,2 I a plasma protein extraction recovery was (96.94 ± 1.05)%, while I in the plasma protein was determined by the intra-day precision <3. O% n = 5). Table 2I recovery of protein in the plasma (n = 5) 2.2I and plasma protein binding of 2.2.1 to determine the equilibrium time of dialysis in the dialysis bag with phosphate buffer instead of plasma, measured by the above method different time bag, bags outside the I concentration. Whichever is the relationship between the concentration ratio of time to do map, get Figure 4. Equilibrium dialysis shows that I is about 50h. l0.80.60.40.200510203040455oh Figure 4 bags and bags outside the H / rIME ratio changes with time 2.2.2 equilibrium dialysis protein binding determination of the bag, outside the I concentrations were measured five times to calculate different concentrations of protein binding. Shown in Table 3. ere was a significant difference (P <0.O1). Table 3 plasma drug concentration and plasma protein binding of I added toluene diisocyanate (I) concentration in mol / L numbers outside the dialysis bag with rate (%) O. 14O. 33O. 58O. 891.181.5288.383.579.375.373.270.8 experimental results show that toluene diisocyanate (rDI) and plasma protein binding rate and concentration. High concentrations, combined with a decline in binding sites become saturated, free I concentration. I with human plasma protein binding studies of occupational exposure were protective care have some reference value. [
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