,uggs Günstig
Dual-wavelength TLC Determination of eugenol content of clove


Reagents were of analytical grade. 1.2 Main apparatus CS-930 dual wavelength TLC scanner, Shimadzu, Japan. Quantitative micro-capillary, the United States Drummondscientific (30.. MettIerToledo hundred thousandth of electronic analytical balance. 1.3 Methods 1.3.1 Preparation of sample solution cloves meal is about 2.0g, accurately weighed, add ether 50rnl, water bath 1h after extraction, water 50rnl, heating steam to the solvent ether layer, add methanol to make into a 50ml, and the supernatant liquid as samples back. 1.3.2 reference substance solution obtained precision that eugenol standard,ugg paris, add methanol to make into a 10rng / r ~ solution, as a reference fluid. 1.3.3 to start with chloroform-methanol-water (6:3:1) lower as the agent: point-like, chromatography plate in a chromatography tank saturated 10min, up started out dry,canada goose jackets, observed under ultraviolet light at 254nm silica gel GF254 chromatography plate, the inlet fluid chromatography, chromatography with reference substance the same as the corresponding position of the dark spots on the star, is O.65, well separated Figure 1 Figure 1 Figure 1 TI.EL skullcap, for the test items 2, standard liquid instrument parameters 1.3.4 dual-wavelength reflection zigzag scanning, = 296nm, = 370nm,moncler paris, slit 1.25mmx1.25mm, the linear coefficient SX = 5, 0.02 Drift lines 2 and the results of the regression equation linear in the range 2.1 points eugenol reference substance 0.5ul, 1.0d, 2.0d, 3.0d, 4.0d, 5.0d the same TLC plate, start scanning in the UV lamp positioned determination of sample volume on the spot to point area value of the standard curve to calculate the regression equation, the results point sample volume 5.0ug--50. 0ug its peak area was within the linear regression equation is Y = 6226.3X +1055.5. r = 0.99983 (n = China Eastern Rui clinical study drug Chi Ji 102 2003 6), see Table 1 Table 1 o'clock and determination of sample volume integral values ​​(n = 6) the amount of fallen Mexican Man:: Pad:::: integral 30959630781B5341 ~ 315 ~ 23023097252.2 stability test to eugenol point of reference substance in methanol solution 2ul thin laminates, expand, dry, repeated measurements at intervals of a certain time integral value spots results show that the eugenol spots in 5h is stable,ralph lauren sale, RSD = 0.13% (n = 8), see Table 2 Table spots within 25h integral values ​​(n = 8) at Austin (030 ∞ 9D1201 midship two handsome 30 active touch 1125567125835125611125 {86,125,615,125,465 2.3 ridicule the same precision of the sample preparation liquid medicine in the same TLC plate points 10 points to start, spots measured integral value, RSD = 0.2% (n = 10), shows good precision, see Table 3 Table 3 the same TLC plate spot integral value (n = 10) # 12345678910 integral tease canthus 70222 butterfly 69856701n70l3569951 bilge (iv) 2.4 Recovery test precision that take different amounts of reference substance eugenol, eugenol content added to the known sample, the sample preparation method according to the aforementioned solution, point-like, expand, according to the determination of their recoveries Results: The average recovery was 98.79%, RSD = 0.48% (n = 5), Table 4 and Table 4, the amount determined by adding the amount of contrast (n = 5) 2.5 Determination of the sample and the sample Preparation of reference substance solution using micro-quantitative capillary samples in the same TLC plate (10cmX10cm), started to dry, to the aforementioned experimental conditions for determination of spot and calculating the eugenol content of the average content of 13.85%, RSD = 0.43% (n = 5), determination results in Table 5 Table 5 Determination of the integral value of the sample (n = 5) 3 has been discussed in preliminary experiments with water - saturated n-butanol, acetic acid-water - saturated butyl alcohol, ether and cyclohexane a different proportion of chloroform - methanol aqueous solution as mobile phase, the final selection of chloroform-methanol-water (6:3:1) solution system for the preferred option to achieve a sample of eugenol completely separated from other components of the effect of this experiment the test solution preparation, extraction, separation step is simple, fast and easy the whole measured by average recovery and precision of experimental results show that the assay method is reliable, accurate, not only can be used to control the quality of medicines, can also be used with cloves of traditional Chinese medicine determination of the corresponding index components.