Interleukin-2 and IFN-α reverse multidrug resistance of leukemia cells


U/m1 pgp -positive rate ) after 24 hours were 94.5,94.2 , did not change significantly . Sister cafe E avoidance training chestnut tenant (Dan ) the circle 2IFNlL 2 DNR concentration in the intracellular K562/AO2 4RT - PCR, seized one side of the mdr 1 mRNA expression in the level of K562 / S and K562/A02 and increase of IFN -n ( 50oU / m1) , IL- 2 ( 250U/m1 ) for 24 hours group there was no significant difference . Description of IFN- n and IL- 2 reversal of drug resistance than through the downward mdrlmRNA mechanism . Figure 3. e circle 3K562fAI ) 2mdr [ mRNA electrophoresis pattern discussion of this study show that the cell IFN - n- ,uggs Günstig, IL- 2 allows drug-resistant cell line K562/A02 DNR sensitivity increase , increase the concentration of the drug resistant cells ,ralph lauren online, with partial reversal of the blood disease cell multi-drug resistant role . But it does not affect the level of mdr 1 mRNA expression and its corresponding product of the amount of p - gp , Chin, ] Hemato [ December1997 , Vol18 , No. 12 it is not through inhibition of mdr -1 mechanism . Of G-CSF , GM - CSF does not change the sensitivity of K562 / S and K562/AO2 to DNR . K562/A02 cell line used in this test the resistance mechanisms of non - single mechanism , including the mdr -1 over- expression, G . P and Topo II adjustment mechanism . Chief among these is ,ralph lauren online shop, of mdr - l over-expression of mdr 1 mRNA expression in increased levels of the cells to produce a membrane glycoprotein (p ​​- gP ) , as an ATP dependent pump into the cells within the chemotherapy pump drugs out of cells , resulting in drug resistance. We speculate that to IFNa ,moncler sale, IL- 2 reversal of drug resistance mechanisms may have the following : ① of IFN- a , IL- 2 pgp function , drug efflux pumps to reduce ; 2 may be associated with other resistance mechanisms , in particular in MRP is also a drug efflux pump . IFNn , IL- 2 may affect the MRP and reversal of drug resistance : ③ e IFN ‰ of IL -2 cytokines may affect tumor cell proliferation and apoptosis with chemotherapy drugs , synergy ; ④ IFNIL a 2 as a cytokine . May affect the tumor cell membrane permeability , fluidity and function . In short, the cytokines IFN , IL- 2 as a multidrug resistance reversal agents with the characteristics of the toxic side effects ,nike free, has broad prospects for clinical application .